sheep red blood cell suspension  (Innovative Research Inc)

Journal: bioRxiv

Article Title: Arid2 promotes Follicular B-cell differentiation and antibody responses in vivo

doi: 10.1101/2025.10.01.678812

Figure Lengend Snippet: a. Schematic of the SRBC immunization protocol b. Total spleen cell counts in mice of indicated genotypes c-d. ELISA measurements of IgM ( b ) and IgG ( c ) antibody concentrations from the peripheral blood of immunized Mb1-Cre and Arid2 F/F Mb1-Cre mice. e. Representative flow cytometry plots of GCB cells from spleens of immunized Mb1-Cre and Arid2 F/F Mb1-Cre mice. f. Flow cytometric analysis of GCB cells in the spleen of immunized mice as a percentage of total live cells. g. Absolute number of GCB cells described in ( e ) calculated from total spleen counts (b). ns – not significant, *p<0.05, **p<0.01 by Welch’s t-test or multiple unpaired t-tests. Error bars indicate standard deviation.

Article Snippet: For germinal center studies, age-matched mice were immunized intraperitoneally between 8–12 weeks of age with 0.5 ml of a 2% sheep red blood cell (SRBC) suspension in PBS (Cocalico biologicals, 20–1334A).

Techniques: Enzyme-linked Immunosorbent Assay, Flow Cytometry, Standard Deviation

Journal: bioRxiv

Article Title: Arid2 promotes Follicular B-cell differentiation and antibody responses in vivo

doi: 10.1101/2025.10.01.678812

Figure Lengend Snippet: a. Reduced spleen weight and cellularity in transplant recipients of Arid2 F/F Mb1-Cre bone marrow after SRBC immunization. (n=5) cells. b. Serum IgM concentrations of recipients of Arid2 F/F Mb1-Cre vs Mb1-Cre control bone marrow. c. Serum IgG concentrations significant reduced in recipients of Arid2 F/F Mb1-Cre marrow. d. Representative flow cytometry plots of GCB cells from spleens of immunized mice transplanted with Mb1-Cre and Arid2 F/F Mb1-Cre cells. e. Flow cytometric analysis of GCB cells in the spleen of immunized transplant mice as a percentage of total live cells. f. Absolute number of GCB cells described in (e) calculated from total spleen counts (a). ns – not significant, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by Welch’s t-test or multiple unpaired t-tests. Error bars indicate standard deviation.

Article Snippet: For germinal center studies, age-matched mice were immunized intraperitoneally between 8–12 weeks of age with 0.5 ml of a 2% sheep red blood cell (SRBC) suspension in PBS (Cocalico biologicals, 20–1334A).

Techniques: Control, Flow Cytometry, Standard Deviation